Review




Structured Review

SignaGen polyjet in vitro dna transfection reagent
Polyjet In Vitro Dna Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+dna+in+vitro+transfection+reagent/polyjet+dna+transfection+reagent/pmc09481576__41467_2022_33111_MOESM1_ESM-205-22-28
Average 90 stars, based on 1 article reviews
polyjet in vitro dna transfection reagent - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

In Vitro:

Article Title: Regulation of glucocorticoid receptor nuclear localization in prostate cancer cells.
Article Snippet: Glucocorticoid receptor (GR) plays important roles in many diseases including prostate cancer.. Intracellular shuttling of GR is thought to be an important mechanism regulating its localization to the nucleus required for transactivation of GR target genes.. Here, using fluorescent microscopy coupled with pulse-chase and nucleocytoplasmic fractionation coupled with western blot, we provided evidence that GR can be imported and then degraded in the nucleus in the absence of ligand.

Article Title: Receptor activity-modifying protein 1 regulates the differentiation of mouse skin fibroblasts by downregulating α-SMA expression via suppression of high mobility group AT-hook 1 to promote skin wound repair
Article Snippet: .. Transfection was performed with PolyJet DNA In Vitro Transfection Reagent (SignaGen, USA) according to the manufacturer’s protocol. ..

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.
Article Snippet: .. Lentiviruses expressing dCas9-VP64, MS2-P65-HSF1, and sgRNAs A-D were generated in 293 T cells by co-transfection of the packaging plasmids psPAX.2 and psMD2.Gusing PolyJet DNA in vitro transfection reagent (SignaGen Laboratories, Rockville, MD). .. Virus-containing supernatant was harvested 48 h post transfection and filtered through a 0.45μm filter (Fisher Scientific, Pittsburgh, PA) followed by concentration using Lenti-X concentrator (Clontech).

Article Title: A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.
Article Snippet: .. Transfections used PolyJet DNA In Vitro Transfection Reagent (SignaGen) with a PolyJet: DNA volume to mass ratio of 3:1 according to manufacturer instructions. ..

Article Title: A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.
Article Snippet: .. HEK293T transfections used PolyJet DNA In Vitro Transfection Reagent (SignaGen) with a PolyJet: DNA volume to mass ratio of 3:1, according to manufacturer instructions. .. C2C12 myoblasts were seeded in a black 96-well plastic bottom plate (VWR) at a density of 5.0 × 103 cells per well twenty-four hours prior to transfection.

Article Title: MiR-146b overexpression promotes bladder cancer cell growth via the SMAD4/C-MYC/Cyclin D1 axis
Article Snippet: T24T cells were cultured with a 1:1 mixture of DMEM/Ham’s F12 medium (Gibco, Grand Island, NY, USA) supplemented with 5% FBS. .. Stable transfections were performed with constructs using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer’s instructions, and stable transfectants were selected with puromycin (0.2–0.3 mg/mL) or hygromycin B (200–400 mg/mL) for 3 or 4 weeks according to the different antibiotic resistance plasmids transfected ( , ). ..

Article Title: Antibody Binding to Recombinant Adeno Associated Virus Monitored by Charge Detection Mass Spectrometry
Article Snippet: .. Empty AAV8 was prepared by transfecting equal molar ratios of pRep2-Cap8 packaging plasmid and mini-adenovirus helper plasmid into HEK 293 cells (human embryonic kidney cells CRL-1573, ATCC) using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories). ..

Transfection:

Article Title: Regulation of glucocorticoid receptor nuclear localization in prostate cancer cells.
Article Snippet: Glucocorticoid receptor (GR) plays important roles in many diseases including prostate cancer.. Intracellular shuttling of GR is thought to be an important mechanism regulating its localization to the nucleus required for transactivation of GR target genes.. Here, using fluorescent microscopy coupled with pulse-chase and nucleocytoplasmic fractionation coupled with western blot, we provided evidence that GR can be imported and then degraded in the nucleus in the absence of ligand.

Article Title: Receptor activity-modifying protein 1 regulates the differentiation of mouse skin fibroblasts by downregulating α-SMA expression via suppression of high mobility group AT-hook 1 to promote skin wound repair
Article Snippet: .. Transfection was performed with PolyJet DNA In Vitro Transfection Reagent (SignaGen, USA) according to the manufacturer’s protocol. ..

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.
Article Snippet: .. Lentiviruses expressing dCas9-VP64, MS2-P65-HSF1, and sgRNAs A-D were generated in 293 T cells by co-transfection of the packaging plasmids psPAX.2 and psMD2.Gusing PolyJet DNA in vitro transfection reagent (SignaGen Laboratories, Rockville, MD). .. Virus-containing supernatant was harvested 48 h post transfection and filtered through a 0.45μm filter (Fisher Scientific, Pittsburgh, PA) followed by concentration using Lenti-X concentrator (Clontech).

Article Title: A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.
Article Snippet: .. Transfections used PolyJet DNA In Vitro Transfection Reagent (SignaGen) with a PolyJet: DNA volume to mass ratio of 3:1 according to manufacturer instructions. ..

Article Title: A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.
Article Snippet: .. HEK293T transfections used PolyJet DNA In Vitro Transfection Reagent (SignaGen) with a PolyJet: DNA volume to mass ratio of 3:1, according to manufacturer instructions. .. C2C12 myoblasts were seeded in a black 96-well plastic bottom plate (VWR) at a density of 5.0 × 103 cells per well twenty-four hours prior to transfection.

Article Title: MiR-146b overexpression promotes bladder cancer cell growth via the SMAD4/C-MYC/Cyclin D1 axis
Article Snippet: T24T cells were cultured with a 1:1 mixture of DMEM/Ham’s F12 medium (Gibco, Grand Island, NY, USA) supplemented with 5% FBS. .. Stable transfections were performed with constructs using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer’s instructions, and stable transfectants were selected with puromycin (0.2–0.3 mg/mL) or hygromycin B (200–400 mg/mL) for 3 or 4 weeks according to the different antibiotic resistance plasmids transfected ( , ). ..

Article Title: Antibody Binding to Recombinant Adeno Associated Virus Monitored by Charge Detection Mass Spectrometry
Article Snippet: .. Empty AAV8 was prepared by transfecting equal molar ratios of pRep2-Cap8 packaging plasmid and mini-adenovirus helper plasmid into HEK 293 cells (human embryonic kidney cells CRL-1573, ATCC) using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories). ..

Recombinant:

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Modification:

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Saline:

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Protease Inhibitor:

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Sequencing:

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry.
Article Snippet: Kaushal et al., STAR Protocols 5, 103286 Proteins congregate into complexes to perform diverse cellular functions.. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease.. We present an affinity purification-mass spectrometry (APMS) proteomics protocol to express affinity-tagged ‘‘bait’’ proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions.

Expressing:

Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.
Article Snippet: .. Lentiviruses expressing dCas9-VP64, MS2-P65-HSF1, and sgRNAs A-D were generated in 293 T cells by co-transfection of the packaging plasmids psPAX.2 and psMD2.Gusing PolyJet DNA in vitro transfection reagent (SignaGen Laboratories, Rockville, MD). .. Virus-containing supernatant was harvested 48 h post transfection and filtered through a 0.45μm filter (Fisher Scientific, Pittsburgh, PA) followed by concentration using Lenti-X concentrator (Clontech).

Generated:

Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.
Article Snippet: .. Lentiviruses expressing dCas9-VP64, MS2-P65-HSF1, and sgRNAs A-D were generated in 293 T cells by co-transfection of the packaging plasmids psPAX.2 and psMD2.Gusing PolyJet DNA in vitro transfection reagent (SignaGen Laboratories, Rockville, MD). .. Virus-containing supernatant was harvested 48 h post transfection and filtered through a 0.45μm filter (Fisher Scientific, Pittsburgh, PA) followed by concentration using Lenti-X concentrator (Clontech).

Construct:

Article Title: MiR-146b overexpression promotes bladder cancer cell growth via the SMAD4/C-MYC/Cyclin D1 axis
Article Snippet: T24T cells were cultured with a 1:1 mixture of DMEM/Ham’s F12 medium (Gibco, Grand Island, NY, USA) supplemented with 5% FBS. .. Stable transfections were performed with constructs using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer’s instructions, and stable transfectants were selected with puromycin (0.2–0.3 mg/mL) or hygromycin B (200–400 mg/mL) for 3 or 4 weeks according to the different antibiotic resistance plasmids transfected ( , ). ..

Plasmid Preparation:

Article Title: Antibody Binding to Recombinant Adeno Associated Virus Monitored by Charge Detection Mass Spectrometry
Article Snippet: .. Empty AAV8 was prepared by transfecting equal molar ratios of pRep2-Cap8 packaging plasmid and mini-adenovirus helper plasmid into HEK 293 cells (human embryonic kidney cells CRL-1573, ATCC) using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories). ..



Similar Products

86
Yeasen Biotechnology polyjet in vitro dna transfection reagent pei
Polyjet In Vitro Dna Transfection Reagent Pei, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+dna+in+vitro+transfection+reagent/hieff+liposomal+reagent+trans+transfection/pm41876625-253-18-26
Average 86 stars, based on 1 article reviews
polyjet in vitro dna transfection reagent pei - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

90
SignaGen polyjet in vitro dna transfection reagent
Polyjet In Vitro Dna Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+dna+in+vitro+transfection+reagent/polyjet+dna+transfection+reagent/pmc09481576__41467_2022_33111_MOESM1_ESM-205-22-28
Average 90 stars, based on 1 article reviews
polyjet in vitro dna transfection reagent - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
SignaGen polyjet dna in vitro transfection reagent
Polyjet Dna In Vitro Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+dna+in+vitro+transfection+reagent/polyjet+dna+transfection+reagent/pm40598087-208-2-8
Average 90 stars, based on 1 article reviews
polyjet dna in vitro transfection reagent - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results